Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit | KTE62342

Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit | KTE62342 | Gentaur UK, US & Europe Distribution

Application: This Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKRIP1 in samples. An antibody specific for PRKRIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKRIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKRIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKRIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human PRKR-interacting protein 1 microplate
• Human PRKR-interacting protein 1 standard
• Human PRKR-interacting protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKRIP1. No significant cross-reactivity or interference between Human PRKRIP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The C114 cDNA contains an open reading frame of 187 amino acids with a predicted mass of 21 kDa. Using green fluorescent protein (GFP) -C114 fusion plasmids, amino acids 126-131 are shown to be essential for the nuclear localization of C114. An arginine-rich region (amino acids 98-143) spanning the nuclear localization signals (amino acids 126-131) exhibits a double-stranded RNA (dsRNA) binding activity. Competition experiments with different RNA homopolymers demonstrate that C114 preferentially binds to poly (I.C) . C114 binds to the dsRNA-activated protein kinase, protein kinase R (PKR), via dsRNA-binding domains of PKR and the N-terminal region of the C114 protein. In vitro kinase assays indicate that C114 inhibits PKR activation via a dsRNA-independent mechanism.

Alternative Names: PRKRIP1; C114; FLJ13902; FLJ40957; likely ortholog of mouse C114 dsRNA-binding protein

Search name: PRKRIP1; C114; FLJ13902; FLJ40957; likely ortholog of mouse C114 dsRNA-binding protein

Tag: PRKRIP1