Description
Human PRMT1 (Protein Arginine Methyltransferase 1) ELISA Kit | ELK5605 | Gentaur UK, US & Europe Distribution
Alternative Names: ANM1; HCP1; HRMT1L2; IR1B4; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4
Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Arginine Methyltransferase 1 (PRMT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Arginine Methyltransferase 1 (PRMT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Arginine Methyltransferase 1 (PRMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Arginine Methyltransferase 1 (PRMT1) in the samples is then determined by comparing the OD of the samples to the standard curve.