Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit | KTE62119

Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit | KTE62119 | Gentaur UK, US & Europe Distribution

Application: This Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit employs a two-site sandwich ELISA to quantitate DDX4 in samples. An antibody specific for DDX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Probable ATP-dependent RNA helicase DDX4 microplate
• Human Probable ATP-dependent RNA helicase DDX4 standard
• Human Probable ATP-dependent RNA helicase DDX4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Probable ATP-dependent RNA helicase DDX4 (DDX4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DDX4. No significant cross-reactivity or interference between Human DDX4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX4 encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. DDX4 is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for DDX4.

Alternative Names: DDX4; MGC111074; VASA; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Search name: DDX4; MGC111074; VASA; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Tag: DDX4