Human Probable carboxypeptidase PM20D1 (PM20D1) ELISA Kit | AE26883HU

Human Probable carboxypeptidase PM20D1 (PM20D1) ELISA Kit | AE26883HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PM20D1

Alternative Name: Cps1; FLJ32569;

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.078 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤7.1%

Recovery: 0, 92

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PM20D1 in samples. An antibody specific for PM20D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPM20D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PM20D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PM20D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PM20D1 belongs to the peptidase M20A family. This domain consists of 4 beta strands and two alpha helices which make up the dimerisation surface of members of the MEROPS peptidase family M20. Loss of activity was due to decreased amounts of aminoacylase I protein. The amount of mRNA for the aminoacylase I was decreased. DNA sequencing of the encoding ACY1 gene showed a homozygous c.1057 C>T transition, predicting a p.Arg353Cys substitution. Both parents were heterozygous for the mutation. The mutation was also detected in 5/161 controls. To exclude the possibility of a genetic polymorphism, protein expression studies were performed showing that the mutant protein had lost catalytic activity.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .