Human Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit | AE61760HU

Human Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit | AE61760HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: APIP

Alternative Name: APIP2; CGI-29; CGI29; MMRP19; dJ179L10.2; APAF1-interacting protein

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 14.5 pg/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤7.3%

Recovery: 1, 07

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate APIP in samples. An antibody specific for APIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APIP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: APIP is an APAF1-interacting protein that acts as a negative regulator of ischemic/hypoxic injury.The deduced APIP protein contains 204 amino acids. A putative alternatively spliced APIP isoform called APIP3 was identified by database analysis and encodes检̩桰̩

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .