Human PROC (Protein C) ELISA Kit | ELK2048

Human PROC (Protein C) ELISA Kit | ELK2048 | Gentaur UK, US & Europe Distribution

Alternative Names: PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide

Species: Human

Assay Type: Sandwich

Sensitivity: 24.8 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Plasma.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Hematology;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C (PROC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein C (PROC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C (PROC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C (PROC) in the samples is then determined by comparing the OD of the samples to the standard curve.