Human Prolyl 3-hydroxylase 2 (LEPREL1) ELISA Kit | KTE61831

Human Prolyl 3-hydroxylase 2 (LEPREL1) ELISA Kit | KTE61831 | Gentaur UK, US & Europe Distribution

Application: This Human Prolyl 3-hydroxylase 2 (LEPREL1) ELISA Kit employs a two-site sandwich ELISA to quantitate LEPREL1 in samples. An antibody specific for LEPREL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLEPREL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LEPREL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LEPREL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Prolyl 3-hydroxylase 2 microplate
• Human Prolyl 3-hydroxylase 2 standard
• Human Prolyl 3-hydroxylase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Prolyl 3-hydroxylase 2 (LEPREL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LEPREL1. No significant cross-reactivity or interference between Human LEPREL1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LEPREL1 belongs to a family of collagen prolyl hydroxylases required for proper collagen biosynthesis, folding, and assembly. The deduced 708-amino acid protein has an N-terminal signal peptide, 4 tetratricopeptide repeats, 4 CxxxC motifs, a central leucine zipper, a prolyl 4-hydroxylase-alpha domain, which contains an ATP/GTP-binding site, and a C-terminal endoplasmic reticulum (ER) retention motif (KDEL) . LEPREL1 also has 3 potential N-glycosylation sites and putative glycosaminoglycan attachment sites. It shares 53% and 45% amino acid identity with LEPRE1 and LEPRE2, respectively. Northern blot analysis detected weak expression of a 3.4-kb transcript in most tissues examined, with higher levels in placenta and kidney. A 6.5-kb transcript was expressed abundantly in skeletal muscle and more weakly in heart.

Alternative Names: LEPREL1; FLJ10718; MLAT4; P3H2; myxoid liposarcoma associated protein 4; prolyl 3-hydroxylase 2; prolyl 3-hydroxylase 3

Search name: LEPREL1; FLJ10718; MLAT4; P3H2; myxoid liposarcoma associated protein 4; prolyl 3-hydroxylase 2; prolyl 3-hydroxylase 3

Tag: LEPREL1