Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit | KTE62360

Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit | KTE62360 | Gentaur UK, US & Europe Distribution

Application: This Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK7 in samples. An antibody specific for PCSK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Proprotein convertase subtilisin/kexin type 7 microplate
• Human Proprotein convertase subtilisin/kexin type 7 standard
• Human Proprotein convertase subtilisin/kexin type 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCSK7. No significant cross-reactivity or interference between Human PCSK7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Proprotein convertase subtilisin/kexin type 7 is an enzyme belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease. It is structurally related to its family members, PACE and PACE4. This protein is concentrated in the trans-Golgi network, associated with the membranes, and is not secreted. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes. Multiple alternatively spliced transcripts are described for this gene but their full length nature is not yet known.

Alternative Names: PCSK7; FLJ23503; FLJ45329; LPC; PC7; PC8; SPC7; lymphoma proprotein convertase; prohormone convertase 8; subtilisin-like; prohormone convertase PC7; proprotein convertase PC7; subtilisin/kexin-like protease PC

Search name: PCSK7; FLJ23503; FLJ45329; LPC; PC7; PC8; SPC7; lymphoma proprotein convertase; prohormone convertase 8; subtilisin-like; prohormone convertase PC7; proprotein convertase PC7; subtilisin/kexin-like protease PC

Tag: PCSK7