Human proteasome (prosome, macropain) subunit beta type 1 (PSMb1) ELISA kit | ELK8540

Human proteasome (prosome, macropain) subunit beta type 1 (PSMb1) ELISA kit | ELK8540 | Gentaur UK, US & Europe Distribution

Alternative Names: PSC5, HC5, Macropain subunit C5; PSMb1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to proteasome subunit beta type 1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to proteasome subunit beta type 1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain proteasome subunit beta type 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of proteasome subunit beta type 1 in the samples is then determined by comparing the OD of the samples to the standard curve.