Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit | AE24050HU

Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit | AE24050HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: ADPRH

Alternative Name: ARH1; ADP-ribose-L-arginine cleaving enzyme

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.059 ng/mL

Intra-Assay: ≤4.1%

Inter-Assay: ≤8.0%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate ADPRH in samples. An antibody specific for ADPRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADPRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADPRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADPRH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The deduced human ADPRH protein has 357 amino acids and an estimated molecular mass of 39.5 kD. Human ADPRH shows 83% amino acid identity to rat Adprh and 82% identity to mouse Adprh. The authors found that the mouse and rat Adprh sequences contain 5 conserved cysteine residues; the human sequence contains only 4 cysteines, with cys108 in rat corresponding to ser103 in human. Expression of recombinant wildtype and mutant rat and human ADPRH proteins in E. coli and assessment of their DTT requirements for hydrolase activity demonstrated that cys108 is responsible for the DTT dependence of rat Adprh. Northern blot analysis detected a 4.1-kb ADPRH transcript in various human tissues, including fetal and adult brain.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .