Human Protein prune homolog 2 (PRUNE2) ELISA Kit | KTE61097

Human Protein prune homolog 2 (PRUNE2) ELISA Kit | KTE61097 | Gentaur UK, US & Europe Distribution

Application: This Human Protein prune homolog 2 (PRUNE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRUNE2 in samples. An antibody specific for PRUNE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRUNE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRUNE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRUNE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Protein prune homolog 2 microplate
• Human Protein prune homolog 2 standard
• Human Protein prune homolog 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Protein prune homolog 2 (PRUNE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRUNE2. No significant cross-reactivity or interference between Human PRUNE2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The deduced 2, 714-amino acid protein has a coiled-coil sequence, a proline-rich region, a P loop, and a C-terminal BNIP2 and CDC42GAP (ARHGAP1) homology (BCH) domain, which contains 3 putative transmembrane domains. Northern blot analysis detected a 12-kb transcript in fetal brain. Semiquantitative RT-PCR revealed BMCC1 expression in all tissues examined except bone marrow, thymus, and spleen. Expression was highest in brain, cerebellum, spinal cord, and adrenal gland. RT-PCR of synchronized HeLa cells showed that BMCC1 was predominantly expressed in G1 phase of the cell cycle. In situ hybridization of mouse embryos showed that Bmcc1 was specifically expressed in neural tube and neural crest-related tissues.

Alternative Names: PRUNE2; RP11-214N16.3; A214N16.3; BMCC1; BNIPXL; C9orf65; DKFZp762K117; FLJ50060; FLJ54876; FLJ59118; KIAA0367; RP11-58J3.2; bA214N16.3; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2

Search name: PRUNE2; RP11-214N16.3; A214N16.3; BMCC1; BNIPXL; C9orf65; DKFZp762K117; FLJ50060; FLJ54876; FLJ59118; KIAA0367; RP11-58J3.2; bA214N16.3; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2

Tag: PRUNE2