Human Protein Wnt-2 (WNT2) ELISA Kit | AE11146HU

Human Protein Wnt-2 (WNT2) ELISA Kit | AE11146HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: WNT2

Alternative Name: INT1L1; IRP; Int-1-related protein|protein Wnt-2|secreted growth factor

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.057 ng/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤6.5%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate WNT2 in samples. An antibody specific for WNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: WNT2 is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins which are involved in the Wnt signaling pathway. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. The INT1-related protein is an additional member of the INT1 growth factor gene family. Because of its homology to protooncogene INT1, IRP is also symbolized INT1L1. IRP is expressed in a variety of fetal and adult human tissues that do not overlap with the pattern of expression of INT1.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .