Human Protein Wnt-3a (WNT3A) ELISA Kit | AE11136HU

Human Protein Wnt-3a (WNT3A) ELISA Kit | AE11136HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: WNT3A

Alternative Name: MGC119418; MGC119419; MGC119420;

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.055 ng/mL

Intra-Assay: ≤4.4%

Inter-Assay: ≤7.9%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate WNT3A in samples. An antibody specific for WNT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT3A is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. WNT3A encodes a deduced 352-amino acid protein containing an N-linked glycosylation site and residues conserved among members of the WNT family. The WNT3A protein shares 84.9% sequence identity with human WNT3 .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .