Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit | AE63109HU

Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit | AE63109HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PIM1

Alternative Name: PIM; Oncogene PIM1|non-specific serine/threonine protein kinase|pim-1 kinase 44 kDa isoform|pim-1 oncogene (proviral integration site 1)

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.27 ng/mL

Intra-Assay: ≤6.3%

Inter-Assay: ≤10.1%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PIM1 in samples. An antibody specific for PIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Proto-oncogene serine/threonine-protein kinase Pim-1 is an enzyme encoded by the PIM1 gene. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001) .Viral leukemogenesis in mice is often initiated by proviral activation of the highly conserved cellular gene Pim1. Domen et al. (1987) compared the nucleotide sequence of human and mouse PIM1 cDNAs. Meeker et al. (1987) also characterized the human PIM1 gene. The deduced amino acid sequence showed significant homology to a number of the protein kinases but did not have a transmembrane region. Studies of expression of this gene using Northern blots of human cell lines showed it to be transcribed primarily in B-lymphoid and myeloid cell lines.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .