Human PXDN (Peroxidasin Homolog) ELISA Kit | ELK6674

Human PXDN (Peroxidasin Homolog) ELISA Kit | ELK6674 | Gentaur UK, US & Europe Distribution

Alternative Names: PRG2; PXN; MG50; VPO; VPO1; Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxidasin Homolog (PXDN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peroxidasin Homolog (PXDN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxidasin Homolog (PXDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxidasin Homolog (PXDN) in the samples is then determined by comparing the OD of the samples to the standard curve.