Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit | AE28196HU

Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (PDHB) ELISA Kit | AE28196HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PDHB

Alternative Name: DKFZp564K0164; PHE1B; Pyruvate dehydrogenase; E1 beta polypeptide

Application: ELISA

Range: 1.56-100 ng/mL

Sensitivity:  0.65 ng/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤8.0%

Recovery: 0, 94

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PDHB in samples. An antibody specific for PDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Koike et al. (1988) cloned and sequenced cDNAs encoding the alpha and the beta subunits. Theoretically, there may be 2 forms of pyruvate dehydrogenase deficiency, one with mutation in the PDHA gene and one with mutation in the PDHB gene. Ho et al. (1988) isolated a 1.5-kb cDNA clone for the beta subunit of E1 from a human liver gamma-gt11 cDNA library using anti-E1 serum. Using a cDNA probe, Olson et al. (1990) demonstrated that the PHE1B gene is located on 3p13-q23. Koike et al. (1990) described the molecular cloning of the entire human PHE1B gene, its characterization by restriction enzyme analysis, and its complete nucleotide sequence. The gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .