Human R-spondin-1 (RSPO1) ELISA Kit | AE21095HU

Human R-spondin-1 (RSPO1) ELISA Kit | AE21095HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RSPO1

Alternative Name: RP11-566C13.1; CRISTIN3; FLJ40906; RSPO; OTTHUMP00000180612|OTTHUMP00000180613|R-spondin1|roof plate-specific spondin

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 5.5 pg/mL

Intra-Assay: ≤5.8%

Inter-Assay: ≤9.3%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RSPO1 in samples. An antibody specific for RSPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRSPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: RSPO1 is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.R-spondin transcripts were observed mainly in central nervous system (CNS) tissues, especially in association with the roof plate structure, in 10- and 12-dpc embryos. Outside the CNS, R-spondin transcripts were detected in forelimb buds after 10 dpc, especially in the body trunk and the proximal posterior part of the anterior limb bud. Expression was also observed in facial parenchyma surrounding the nasal cavity at 12 dpc.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .