Human Rac GTPase-activating protein 1 (RACGAP1) ELISA Kit | AE24551HU

Human Rac GTPase-activating protein 1 (RACGAP1) ELISA Kit | AE24551HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RACGAP1

Alternative Name: HsCYK-4; ID-GAP; MgcRacGAP; GTPase activating protein

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Intra-Assay: ≤6.4%

Inter-Assay: ≤8.9%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RACGAP1 in samples. An antibody specific for RACGAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRACGAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RACGAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RACGAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The predicted 527-amino acid RACGAP1 protein has a large N-terminal region containing a protein kinase C -like cysteine-rich motif. The authors determined that RACGAP1 shares highest homology with the Drosophila RnRacGAP and the chimerins of rat and human. Functional analysis showed that the GAP domain of RACGAP1 exhibits strong GAP activity towards CDC42, RAC1, and RAC2. Northern blot analysis detected an approximately 3.2-kb RACGAP1 transcript that was most abundantly expressed in testis, with low expression in most other tissues. Western blot analysis detected a RACGAP1 protein of 58 kD in testis extracts. In situ hybridization showed that RACGAP1 expression is restricted to germ cells in mature testis.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .