Human Ras-related protein Rab-3B (RAB3B) ELISA Kit | AE24691HU

Human Ras-related protein Rab-3B (RAB3B) ELISA Kit | AE24691HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RAB3B

Alternative Name: Brain antigen RAB3B

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 3.9 pg/mL

Intra-Assay: ≤6.4%

Inter-Assay: ≤10.3%

Recovery: 0, 95

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RAB3B in samples. An antibody specific for RAB3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding proteins involved in the regulation of secretion.RAB3B protein shares 78% identity with RAB3A. After endocytosis, the PIGR-dIgA complex traverses several endosomal compartments and is exocytosed at the apical plasma membrane Rab3b localized to vesicular structures containing Pigr in canine kidney cells and that GTP-bound Rab3b interacted directly with the cytoplasmic domain of Pigr. Binding of dIgA to Pigr dissociated Rab3b, but a constitutively active Rab3b mutant maintained its interaction with Pigr in dIgA-treated cells. Furthermore, GTP-locked Rab3b inhibited dIgA-stimulated transcytosis.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .