Description
Human RCAN1 (Regulator Of Calcineurin 1) ELISA Kit | ELK5182 | Gentaur UK, US & Europe Distribution
Alternative Names: ADAPT78; CSP1; DSC1; DSCR1; MCIP1; Down Syndrome Critical Region Gene 1; Calcipressin-1; Myocyte-enriched calcineurin-interacting protein 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of Calcineurin 1 (RCAN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Regulator Of Calcineurin 1 (RCAN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of Calcineurin 1 (RCAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of Calcineurin 1 (RCAN1) in the samples is then determined by comparing the OD of the samples to the standard curve.