Human Recombinant AK1 | 6385

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

6385 | Human Recombinant AK1 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant AK1

Alternates names: Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase.

Taglines: Essential for maintenance and cell growth

NCBI Gene ID #: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity: > 6.0 units/ml.

Results: >6.0 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 2.0 µmoles of ADP to ATP + AMP per minute at pH 7.5 at 25C.

Molecular Weight: 23.7 kDa (214 aa, 1-194 aa + NT His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK