Human Recombinant AKR7A2 | 6335

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to Gamma-Hydroxybutyrate in human brain.

6335 | Human Recombinant AKR7A2 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant AKR7A2

Alternates names: Aldo-keto reductase family 7, member A 2, Aflatoxin B1 aldehyde reductase member 2, AFAR, AFAR1, AFB1-AR1, AKR7.

Taglines: Involved in in the detoxification of aldehydes and ketones

NCBI Gene ID #: 16153

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P18893

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is approximately 0.25 – 0.3 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: Specific activity is approximately 0.25 – 0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C. Specific activity was expressed as units/mg protein.

Molecular Weight: 44 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELE LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR