Human Recombinant DUSP3 | 6371

DUSP3 belongs to the dual specificity protein phosphatase subfamily which inactivates their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSP3 negatively regulate members of the mitogen-activated protein kinase superfamily that are related to cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues and displays activity both for tyrosine-protein phosphate and serine-protein phosphate, but exhibits a strong preference toward phosphotyrosines, specifically dephosphorylates and inactivates ERK1 and ERK2.

6371 | Human Recombinant DUSP3 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant DUSP3

Alternates names: Dual specificity protein phosphatase 3, VHR.

Taglines: Negatively regulates MAPK superfamily members

NCBI Gene ID #: 3596

NCBI Gene Symbol: IL13

Gene Source: Human

Accession #: P35225

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity: >1750 units/mg.

Results: >1750 units/mg

Binding Capacity: N/A

Unit Definition: One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.

Molecular Weight: 22.6 kDa (205 aa, 1-185 aa + NT His-Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT and 0.15 M NaCl.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP