Human Recombinant FKBP1a/FKBP12 | 6340

FKBP1A is part of the family of immunophilins, which have in common high affinity for immunosuppressant drugs and a peptidyl-prolyl cis-trans isomerase (PPIase). In the absence of immunosuppressive ligands, FKBP1A is involved in intracellular calcium regulation by associating with 3 types of Ca2+ release channel complexes: skeletal ryanodine receptors, cardiac ryanodine receptors and the inositol 1, 4, 5-triphosphate receptor. FKBP1A also interacts with TGF type I receptor exerting an inhibitory effect on the TGF-I signaling pathway. FKBP12 plays a role in modulation of ryanodine receptor isoform-1 (ryr-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. FKBP1A increase the folding of proteins and catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

6340 | Human Recombinant FKBP1a/FKBP12 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant FKBP1a/FKBP12

Alternates names: FK506 binding protein 1a, 12kDa, PPIase FKBP1A, Rotamase, FKBP1, PKC12

Taglines: An immunophilin family protein functioning as a protein folding chaperone

NCBI Gene ID #: 16163

NCBI Gene Symbol: IL13

Gene Source: Human

Accession #: P20109

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method).

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 300 nmoles/min/mg,

Results: N/A

Binding Capacity: N/A

Unit Definition: Specific activity is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.

Molecular Weight: 14.1 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE