Human Recombinant Fumarase | 6384

Fumarase (Fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.

6384 | Human Recombinant Fumarase DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant Fumarase

Alternates names: Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCUL1.

Taglines: Involved in the TCA or Krebs cycle

NCBI Gene ID #: 3600

NCBI Gene Symbol: IL15

Gene Source: Human

Accession #: P40933

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 1.0 unit/mg

Results: >1.0 unit/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 µmole of L-Malate to Fumarate per minute at pH 7.5 at 25°C.

Molecular Weight: 50.2 kDa (467 aa, 44-510 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0).

Reconstitution Instructions: N/A

Amino acid sequence: MASQNSFRIE YDTFGelKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGelGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GelILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK