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Human Recombinant Fumarase | 6384
- SKU:
- 26-6384-GEN
- Availability:
- Usually shipped in 5 working days
Description
Fumarase (Fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor.
6384 | Human Recombinant Fumarase DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant Fumarase
Alternates names: Fumarate hydratase, FH, HLRCC, LRCC, MCL, MCUL1.
Taglines: Involved in the TCA or Krebs cycle
NCBI Gene ID #: 3600
NCBI Gene Symbol: IL15
Gene Source: Human
Accession #: P40933
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: Specific activity is > 1.0 unit/mg
Results: >1.0 unit/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 µmole of L-Malate to Fumarate per minute at pH 7.5 at 25°C.
Molecular Weight: 50.2 kDa (467 aa, 44-510 aa)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0).
Reconstitution Instructions: N/A
Amino acid sequence: MASQNSFRIE YDTFGelKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGelGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GelILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK