Human Recombinant Neuron-Specific Enolase (NSE) | 6362

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely - neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.

6362 | Human Recombinant Neuron-Specific Enolase NSE DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant Neuron-Specific Enolase (NSE)

Alternates names: Enolase 2 (gamma, neuronal), ENO2.

Taglines: A useful tumor marker for lung cancer patients

NCBI Gene ID #: 112744

NCBI Gene Symbol: IL24

Gene Source: Human

Accession #: Q96PD4

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1.0 EU per 1 g of protein (determined by LAL method).

Activity (Specifications/test method): N/A

Biological activity: Specific activity: >8 units/ml.

Results: >8 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of 2-phosphoglycerate to phosphoenol pyruvate per minute at pH 7.5 at 25°C.

Molecular Weight: 47 kDa (434 aa, 1-434 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl (pH 7.5) containing 100 mM KCl and 5 mM MgSO4.

Reconstitution Instructions: N/A

Amino acid sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL