Human Recombinant PIN1 | 6304

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like -Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

6304 | Human Recombinant PIN1 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant PIN1

Alternates names: Protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, NIMA-interacting protein 1, DOD, UBL5, Rotamase Pin1, PPIase Pin1.

Taglines: Plays a role as a post phosphorylation control in regulating protein function

NCBI Gene ID #: 112744

NCBI Gene Symbol: IL17F

Gene Source: Human

Accession #: Q96PD4

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: > 330 nmoles/min/mg

Results: N/A

Binding Capacity: N/A

Unit Definition: Specific activity is defined as the amount of enzyme that cleaves 1 µmole of SucAAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin

Molecular Weight: 18.2 kDa (163 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5 mM DTT, 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE