Human Recombinant PKLR | 6373

PKLR is a pyruvate kinase which catalyzes the trans phosphorylation of Phosphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. The L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.

6373 | Human Recombinant PKLR DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant PKLR

Alternates names: Pyruvate kinase isozyme R/L, PK1, PKL, PKR, PKRL, RPK.

Taglines: Catalyzes the rate-limiting step of glycolysis

NCBI Gene ID #: 16171

NCBI Gene Symbol: IL17

Gene Source: Human

Accession #: Q62386

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity: >0.1 unit/mg.

Results: >0.1 unit/mg

Binding Capacity: N/A

Unit Definition: One unit will form 1.0 µmol of phosphoenolpyruvate to pyruvate per minute at pH 7.5 at 37°C.

Molecular Weight: 59.2 kDa (549 aa, 47-574 aa + NT His-Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 1 mM DTT and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS