Human Recombinant SHP-1 | 6302

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC Gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.

6302 | Human Recombinant SHP-1 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant SHP-1

Alternates names: Protein tyrosine phosphatase, non-receptor type 6 isoform 1, PTPN6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1L, SHP1.

Taglines: A signaling molecule that regulates a variety of cellular processes

NCBI Gene ID #: 3606

NCBI Gene Symbol: IL18

Gene Source: Human

Accession #: Q14116

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: >5,000 units/mg of SHP-1.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate (pNPP) per minute at pH 7.4 at 37°C using 10 mM of substrate.

Molecular Weight: 34.3 kDa (300 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM -mercaptoethanol, 1 mM EDTA, 20% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY