Description
The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC Gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.
6302 | Human Recombinant SHP-1 DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant SHP-1
Alternates names: Protein tyrosine phosphatase, non-receptor type 6 isoform 1, PTPN6, HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1L, SHP1.
Taglines: A signaling molecule that regulates a variety of cellular processes
NCBI Gene ID #: 3606
NCBI Gene Symbol: IL18
Gene Source: Human
Accession #: Q14116
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)
Activity (Specifications/test method): N/A
Biological activity: >5,000 units/mg of SHP-1.
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate (pNPP) per minute at pH 7.4 at 37°C using 10 mM of substrate.
Molecular Weight: 34.3 kDa (300 aa)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM -mercaptoethanol, 1 mM EDTA, 20% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY