Human recombinant Ubiquitin Activating Enzyme E1 | 6429

UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.

6429 | Human recombinant Ubiquitin Activating Enzyme E1 DataSheet

Biomolecule/Target: N/A

Synonyms: Human recombinant Ubiquitin Activating Enzyme E1

Alternates names: UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2

Taglines: First enzyme involved in ubiquitin conjugation pathway, conjugating ubiquitin to the substrate.

NCBI Gene ID #: 9235

NCBI Gene Symbol: IL32-

Gene Source: Human

Accession #: P24001

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Human IL-32 activity is measured via the dose-dependent induction of TNF- in the human THP-1 monocytic cell line.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 118 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A