Human Recombinant Ubiquitin-K0 | 6498

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines. This molecule will not participate in polyubiquitin formation, and can therefore be used in control experiments for the study of ubiquitylation. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

6498 | Human Recombinant Ubiquitin-K0 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant Ubiquitin-K0

Alternates names: dasC, fipA, tsnC

Taglines: Pure Recombinant Human Ubiquitin-K0

NCBI Gene ID #: 949054

NCBI Gene Symbol: TRXB

Gene Source: E. Coli

Accession #: P0A9P4

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 85%

Assay: SDS-PAGE

Purity: 95%

Assay #2: RP-HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is 16-18 A650/min/mg

Results:

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 12.9 kDa

Concentration: 4 mg/ml

Appearance: Liquid

Physical form description: 4 mg/ml in PBS.

Reconstitution Instructions: N/A

Amino acid sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLAGS