Human Recombinant UCHL3 | 6358

Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. UCH-L3 is a member of the lower molecular weight group of UCHs involved in the hydrolysis of small C-terminal derivatives of ubiquitin that form non-specifically during the process of protein ubiquitinylation. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases.

6358 | Human Recombinant UCHL3 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant UCHL3

Alternates names: Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3

Taglines: A deubiquitinating enzyme

NCBI Gene ID #: 9235

NCBI Gene Symbol: IL32-

Gene Source: Human

Accession #: P24001

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Human IL-32 activity is measured via the dose-dependent induction of TNF- in the human THP-1 monocytic cell line.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 28.3 kDa (250 aa, 1-230 aa + NT His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, and 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA