Description
Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. UCH-L3 is a member of the lower molecular weight group of UCHs involved in the hydrolysis of small C-terminal derivatives of ubiquitin that form non-specifically during the process of protein ubiquitinylation. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases.
6358 | Human Recombinant UCHL3 DataSheet
Biomolecule/Target: N/A
Synonyms: Human Recombinant UCHL3
Alternates names: Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3
Taglines: A deubiquitinating enzyme
NCBI Gene ID #: 9235
NCBI Gene Symbol: IL32-
Gene Source: Human
Accession #: P24001
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Human IL-32 activity is measured via the dose-dependent induction of TNF- in the human THP-1 monocytic cell line.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 28.3 kDa (250 aa, 1-230 aa + NT His Tag)
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA