Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit | AE28041HU

Human Retrotransposon-derived protein PEG10 (PEG10) ELISA Kit | AE28041HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PEG10

Alternative Name: Edr; HB-1; KIAA1051; MEF3L; Mar2; Mart2; RGAG3; MEF3 like 1|embryonal carcinoma differentiation regulated|retrotransposon gag domain containing 3

Application: ELISA

Range: 18.75-1200 pg/mL

Sensitivity: 4.69 pg/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤7.6%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PEG10 in samples. An antibody specific for PEG10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEG10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEG10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEG10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: This gene includes two overlapping reading frames of the same transcript encoding distinct isoforms. The shorter isoform has a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons, and it functions in part by interacting with members of the TGF-beta receptor family. The longer isoform has the active-site DSG consensus sequence of the protease domain of pol proteins. The longer isoform is the result of -1 translational frameshifting that is also seen in some retroviruses. Expression of these two isoforms only comes from the paternal allele due to imprinting. Increased gene expression (as observed by an increase in mRNA levels) is associated with hepatocellular carcinomas PEG10 is a paternally expressed imprinted gene that is expressed in adult and embryonic tissues.Most notable expression occurs in the placenta. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) . This gene has been reported to play a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. In preeclampsia placental tissue, PEG10 has been shown to be downregulated[8] and upregulated[9] implicating it as a possible causal role in the occurrence of preeclampsia.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .