Human RHD (Rh Blood Group, D Antigen) ELISA Kit | ELK5410

Human RHD (Rh Blood Group, D Antigen) ELISA Kit | ELK5410 | Gentaur UK, US & Europe Distribution

Alternative Names: CD240D; Rh30a; Rh4; RhPI; RhII; DIIIc; Rhesus D antigen; Rh polypeptide 2

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.92 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rh Blood Group, D Antigen (RHD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rh Blood Group, D Antigen (RHD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rh Blood Group, D Antigen (RHD) in the samples is then determined by comparing the OD of the samples to the standard curve.