Human Ribonucleases P/MRP protein subunit POP1 (POP1) ELISA Kit | AE26589HU

Human Ribonucleases P/MRP protein subunit POP1 (POP1) ELISA Kit | AE26589HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: POP1

Alternative Name: KIAA0061; MGC17365; processing of precursor 1|processing of precursors 1

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.039 ng/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤7.5%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate POP1 in samples. An antibody specific for POP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: POP1 is a protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. This protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing.Human POP1 encodes a predicted 1, 024-amino acid protein that has a pI of 9.86. On Northern blots, human POP1 is expressed as a major 4.1-kb and minor 6-kb transcript, perhaps due to alternative polyadenylation signals. Anti-POP1 antibodies recognize a doublet of approximately 115 kD on Western blots. In HeLa cells, human POP1 localizes to the nucleus and strongly accumulates in the nucleolus. In co-immunoprecipitation studies, POP1 was associated with both RNase P and RNase MRP snRNAs, and with RNase P enzymatic activity.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .