Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit | AE60301HU

Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit | AE60301HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: NOVA2

Alternative Name: ANOVA; NOVA3; neuro-oncological ventral antigen 3

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Intra-Assay: ≤6.1%

Inter-Assay: ≤10.1%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate NOVA2 in samples. An antibody specific for NOVA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOVA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOVA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOVA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The deduced 492-amino acid protein is 99% identical to the mouse protein and 75% identical to NOVA1, with 98% identity in the 3 RNA-binding KH domains. Western blot analysis showed strong expression of a 75-kD protein in mouse brain, with much lower expression in lung and no expression in other tissues. Functional analysis showed that both Nova1 and Nova2 bind to similar RNA ligands with high affinity. This polymorphism has a dramatic effect on the proportions of neonate and adult alternative transcripts of SCN1A in adult brain tissue, and the effect of the polymorphism appeared to be modified by NOVA2 expression levels. No effect was observed of NOVA2 on the alternative splicing of 17 other neuronally expressed genes.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .