Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit | KTE62364

Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit | KTE62364 | Gentaur UK, US & Europe Distribution

Application: This Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit employs a two-site sandwich ELISA to quantitate NOVA2 in samples. An antibody specific for NOVA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOVA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOVA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOVA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human RNA-binding protein Nova-2 microplate
• Human RNA-binding protein Nova-2 standard
• Human RNA-binding protein Nova-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human RNA-binding protein Nova-2 (NOVA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human NOVA2. No significant cross-reactivity or interference between Human NOVA2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The deduced 492-amino acid protein is 99% identical to the mouse protein and 75% identical to NOVA1, with 98% identity in the 3 RNA-binding KH domains. Western blot analysis showed strong expression of a 75-kD protein in mouse brain, with much lower expression in lung and no expression in other tissues. Functional analysis showed that both Nova1 and Nova2 bind to similar RNA ligands with high affinity. This polymorphism has a dramatic effect on the proportions of neonate and adult alternative transcripts of SCN1A in adult brain tissue, and the effect of the polymorphism appeared to be modified by NOVA2 expression levels. No effect was observed of NOVA2 on the alternative splicing of 17 other neuronally expressed genes.

Alternative Names: NOVA2; ANOVA; NOVA3; neuro-oncological ventral antigen 3

Search name: NOVA2; ANOVA; NOVA3; neuro-oncological ventral antigen 3

Tag: NOVA2