Human RORa (RAR Related Orphan Receptor Alpha) ELISA Kit | ELK5292

Human RORa (RAR Related Orphan Receptor Alpha) ELISA Kit | ELK5292 | Gentaur UK, US & Europe Distribution

Alternative Names: ROR1; ROR2; NR1F1; ROR3; RZRA; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Alpha (RORa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Alpha (RORa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Alpha (RORa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAR Related Orphan Receptor Alpha (RORa) in the samples is then determined by comparing the OD of the samples to the standard curve.