Human RPS6Kb1 (Ribosomal Protein S6 Kinase Beta 1) ELISA Kit | ELK6528

Human RPS6Kb1 (Ribosomal Protein S6 Kinase Beta 1) ELISA Kit | ELK6528 | Gentaur UK, US & Europe Distribution

Alternative Names: PS6K; S6K; S6K1; STK14A; p70 (S6K) -alpha; p70-S6K; p70-alpha; P70-S6 Kinase 1; 70 kDa ribosomal protein S6 kinase 1; Serine/threonine-protein kinase 14A

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) in the samples is then determined by comparing the OD of the samples to the standard curve.