Human RSPO3 (R-Spondin 3) ELISA Kit | ELK6288

Human RSPO3 (R-Spondin 3) ELISA Kit | ELK6288 | Gentaur UK, US & Europe Distribution

Alternative Names: CRISTIN1; PWTSR; THSD2; Thrombospondin, Type I, Domain Containing 2; Protein with TSP type-1 repeat; Roof plate-specific spondin-3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 3 (RSPO3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to R-Spondin 3 (RSPO3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 3 (RSPO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 3 (RSPO3) in the samples is then determined by comparing the OD of the samples to the standard curve.