Human Ryanodine receptor 1 (RYR1) ELISA Kit | AE20951HU

Human Ryanodine receptor 1 (RYR1) ELISA Kit | AE20951HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RYR1

Alternative Name: CCO; MHS; MHS1; RYDR; RYR; SKRR; central core disease of muscle|ryanodine receptor type1|sarcoplasmic reticulum calcium release channel|skeletal muscle ryanodine receptor|type 1-like ryanodine recep

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.28 ng/mL

Intra-Assay: ≤6.2%

Inter-Assay: ≤8.6%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RYR1 in samples. An antibody specific for RYR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRYR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RYR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RYR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Ryanodine receptor 1 is a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described. Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.Skeletal muscle and brain (cerebellum and hippocampus) .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .