Human Ryanodine receptor 2 (RYR2) ELISA Kit | AE20948HU

Human Ryanodine receptor 2 (RYR2) ELISA Kit | AE20948HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: RYR2

Alternative Name: RP4-626J7.1; ARVC2; ARVD2; VTSIP; cardiac muscle ryanodine receptor|cardiac muscle ryanodine receptor-calcium release channel

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.107 ng/mL

Intra-Assay: ≤6.1%

Inter-Assay: ≤9.2%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RYR2 in samples. An antibody specific for RYR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRYR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RYR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RYR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Ryanodine receptors (RyRs) form a class of intracellular calcium channels in various forms of excitable animal tissue like muscles and neurons. It is the major cellular mediator of calcium-induced calcium release (CICR) in animal cells. Ryanodine receptor 2 is a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with catecholaminergic polymorphic ventricular tachycardia, stress-induced polymorphic ventricular tachycardia, and arrhythmogenic right ventricular dysplasia.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .