Human SCFR (Stem Cell Factor Receptor) ELISA Kit | ELK1182

Human SCFR (Stem Cell Factor Receptor) ELISA Kit | ELK1182 | Gentaur UK, US & Europe Distribution

Alternative Names: CD117; KIT; C-Kit; PBT; p145 c-Kit; C-Kit Receptor; Tyrosine-Protein Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Piebald Trait

Species: Human

Assay Type: Sandwich

Sensitivity: 0.61 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor Receptor (SCFR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Stem Cell Factor Receptor (SCFR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stem Cell Factor Receptor (SCFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stem Cell Factor Receptor (SCFR) in the samples is then determined by comparing the OD of the samples to the standard curve.