Human SCG5 (Secretogranin V) ELISA Kit | ELK3704

Human SCG5 (Secretogranin V) ELISA Kit | ELK3704 | Gentaur UK, US & Europe Distribution

Alternative Names: SCGV; P7B2; SGNE1; SgV; 7B2 Protein; Neuroendocrine protein 7B2; Prohormone Convertase Chaperone; Pituitary polypeptide; Secretory granule endocrine protein I

Species: Human

Assay Type: Sandwich

Sensitivity: 3.5 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin V (SCG5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Secretogranin V (SCG5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretogranin V (SCG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretogranin V (SCG5) in the samples is then determined by comparing the OD of the samples to the standard curve.