Human Serine protease 23 (PRSS23) ELISA Kit | KTE61107

Human Serine protease 23 (PRSS23) ELISA Kit | KTE61107 | Gentaur UK, US & Europe Distribution

Application: This Human Serine protease 23 (PRSS23) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS23 in samples. An antibody specific for PRSS23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS23 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS23 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Serine protease 23 microplate
• Human Serine protease 23 standard
• Human Serine protease 23 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Serine protease 23 (PRSS23) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS23. No significant cross-reactivity or interference between Human PRSS23 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRSS23 encodes a member of the trypsin family of serine proteases. Mouse studies found a decrease of mRNA levels after ovulation was induced. This gene seems to be highly conserved in vertebrates and may be an important ovarian protease. PRSS23 is associated with cardiovascular diseases, hematological diseases, neurological diseases, cancer, endocrinological diseases, and urological diseases. The invention also features compounds which bind to and/or activate or inhibit the activity of PRSS23 as well as pharmaceutical compositions comprising such compounds. The invention also provides PRSS23 as a biomarker for diseases such as cardiovascular diseases, hematological diseases, neurological diseases, cancer, endocrinological diseases, and urological diseases.

Alternative Names: PRSS23; MGC5107; SIG13; SPUVE; ZSIG13; serine protease; umbilical endothelium

Search name: PRSS23; MGC5107; SIG13; SPUVE; ZSIG13; serine protease; umbilical endothelium

Tag: PRSS23