Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit | AE61153HU

Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit | AE61153HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: ERN1

Alternative Name: FLJ30999; IRE1; IRE1P; MGC163277; MGC163279; ER to nucleus signalling 1|endoplasmic reticulum to nucleus signalling 1|inositol-requiring 1|protein kinase/endoribonuclease

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.059 ng/mL

Intra-Assay: ≤5.4%

Inter-Assay: ≤8.9%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate ERN1 in samples. An antibody specific for ERN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyERN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Serine/threonine-protein kinase/endoribonuclease IRE1 is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .