Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit | KTE62399

Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit | KTE62399 | Gentaur UK, US & Europe Distribution

Application: This Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit employs a two-site sandwich ELISA to quantitate ERN1 in samples. An antibody specific for ERN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyERN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Serine/threonine-protein kinase/endoribonuclease IRE1 microplate
• Human Serine/threonine-protein kinase/endoribonuclease IRE1 standard
• Human Serine/threonine-protein kinase/endoribonuclease IRE1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ERN1. No significant cross-reactivity or interference between Human ERN1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Serine/threonine-protein kinase/endoribonuclease IRE1 is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Alternative Names: ERN1; FLJ30999; IRE1; IRE1P; MGC163277; MGC163279; ER to nucleus signalling 1; endoplasmic reticulum to nucleus signalling 1; inositol-requiring 1; protein kinase/endoribonuclease

Search name: ERN1; FLJ30999; IRE1; IRE1P; MGC163277; MGC163279; ER to nucleus signalling 1; endoplasmic reticulum to nucleus signalling 1; inositol-requiring 1; protein kinase/endoribonuclease

Tag: ERN1